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3d printed water-soluble scaffolds for rapid production of pdms micro-fluidic flow chambers

by:Tuowei     2019-08-21
We\'re covering a new kind of manufacturing-dimensional (3D)
Bio-compatible Micro
Fluid flow chamber in polydione (PDMS)by 3D-printing water-
Soluble poly (alcohol (PVA)
As a filament of the main scaffold.
The bracket is first embedded in silicone rubber and then remains-
Dissolved freely in water, leaving the inscription of the bracket in hardened silicone rubber.
We demonstrated the advantages of our approach using a normal, inexpensive 3D printer and evaluated the inscription process and channels
Fluid properties using image analysis and digital holographic microscopy.
In addition, we provide a protocol that allows direct printing on coverslips, and we show that flow chambers with channel cross sections below 40 μm × 300 μm can be achieved in 60 minutes
These flow channels are completely transparent and biocompatible and can be used for microscopic applications without further processing.
The protocol we propose promotes simple, fast and adaptable production of microcomputers
Design of fluid channel with high cost
Effective, no specialized training is required and can be used for various cell and bacterial analyses.
Help readers reproduce our Micro
We offer a fully prepared solution, 3D-
Print the channel stand and our custom CAD file
Built-in molding equipment for 3D printer
Flat panel instructions, G-code. Lab-on-a-chip (LOC)
Polydione is usually used for equipment (PDMS)
Thanks to the many ideal properties offered by silicone rubber: easy to use, cheap production, quick integration of pipes, high transparency, air
Permeability and biological compatibility
Traditionally, these devices are produced using soft exposure technology by using the required micro-Fluid channel.
In the manufacturing process, silicone rubber is molded on the silicon motherboard.
This molding step transfers the pattern of the master to the elastic material, then the elastic material can peel off the master and seal it using a glass substrate to produce microfluidic device.
However, these methods often require specialized expensive equipment and training, which makes this method inaccessible and slow in LOC device manufacturing.
A new and easier to understand LOC prototype technology is three. dimensional (3D)printing. 3D-
Printing cost-
Efficient and adaptable Micro
Flow control equipment, with the release of commercial consumers
The development of 3D printers has brought new possibilities.
The most common, cheapest and simple 3D printer type is the fusion deposit modeling printer (FDM).
These printers work by depositing layers of molten plastic to build 3D objectsby-layer. However, micro-
Fluid devices manufactured using FDM printers have many disadvantages, such as irregular channel shapes and channel surfaces, unreliable channel size repeatability, and poor optical transparency, which greatly reduces their potential in microscopic applications
In addition, the poor properties of commonly used thermoplastic plastics, such as unknown biological compatibility and limited air
Permeability is problematic for experiments involving cells or tissues.
In order to overcome the problem of cell or tissue analysis, more complex methods are used to create micro-
Fluid devices using FDM printers have been developed.
Instead of making the whole micro
The fluid device that flows out of the thermoplastic plastic, only the channel bracket uses acrylic-Ding benzene (ABS)
Plastic or heterogeneous as printed material.
Subsequently, the print piece is embedded in silicone rubber or epoxy resin and dissolved with acetone (ABS)or water (isomalt)
, Leaving the mark of the channel bracket in hardened silicone rubber or epoxy resin.
Using this manufacturing process, Micro
With reasonable biological compatibility and air-
Penetration rate can be achieved.
However, the problem of poor optical transparency and irregular channel shape remains unresolved.
In addition, the removal of the ABS channel scaffold involves a strong solvent (acetone)
Sugar printers are not commercially available, making them unavailable to a wide range of research communities.
In this article, we have implemented bio-compatible micro-
Fluid device with predictable shape by embedding 3D printed water-
Dissolve the channel holder in silicone rubber using a regular FDM 3D printer.
To this end, we provide detailed manufacturing protocols for the high pressure silicone rubber flow chamber and silicone rubber flow chamber on the coverslip.
Our micro
By carefully checking the shape, optical transparency, surface roughness and physical channel size deviation of the fluid device between CAD design and print output.
In addition, we show that we can print a reproducible channel bracket with a cross section of 40 × 300 m.
By using a digital holographic microscope experiment to determine the fluid velocity distribution in our flow chamber, we confirm the predicted micro-
Fluid properties of our equipment.
We also provided details about the flat building
Plates set up by our 3D printer and slicing software, this information is rarely released, but it is critical to successfully replicate our proposed protocol. Our micro-
Cheap fluid device, fast production speed, optical transparency, bio-compatible, air
Permeable, repeatable and adjustable, allowing the reader to change the channel design to meet their experimental requirements.
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